Widefield Systems

Zeiss ApoTome (a.k.a. The Legendary)

 

Description: 

Upright epifluorescence AxioImager 2 microscope equipped with an Apotome.2 for optical sectioning.
 

Capabilities:

  Superior background noise reduction by optical sectioning with an Apotome.2

  Encoding: read-out magnification, illumination and contrast settings, and automatic

transfer to ZEN Imaging software

  Motorization for reproducible component settings and automated processes

  Excellent optics and homogeneous illumination in both transmitted light and fluorescence

applications

  Research microscope with high performance focus for maximum precision and 24/7

operation.

  Smart control concept for ergonomic working conditions and multi-user management for

up to ten different users or working scenarios

 

Illumination:

Halogen transmitted light source

Xenon arc lamp (UV, Blue and Green excitation)

Colibri – LED (Red excitation)

 

Objectives:

10x/0.5

20x/0.75

63x/1.4 (O)

10x/0.3 (W)

20x/ 0.5 (W)

40x/1.0 (W)

 

Filter cubes: DAPI, FITC/GFP, TRITC/RFP, Cy5

 

Camera: Zeiss AxioCam HRM 


Software: Zen 2.0

Zeiss Motorized AxioVert (a.k.a. The Motormaster)

 

Description:

Zeiss Axiovert 200 inverted microscope

 

Capabilities:

  The sturdy stand provides attachment possibilities for various tools (micromanipulation)

  Live cell imaging

  Motorized ASI stage

  Inverted configuration

  3D z-stacks

  Stitching

  Multi-position time lapse

 

Illumination:

Halogen transmitted light source and X-cite LED

 

Objectives:

5x/0.15

10x/0.5

20x/0.75

40x/1.3

63x/1.4 (O)

 

Cameras:

Hamamatsu Orca R2

 

Filter Cubes: DAPI, FITC/GFP, TRITC/RFP, Cy5

 

Software: Zen 2.0

 

Stage: Motorized ASI stage

 

Enviromental Control: LCI-CU 501 (temp) & LCI-FC 5N incubation (air/CO2 mixer)

Leica Widefield

 

Description:

Inverted epifluorescence microscope DM IRE2 can incorporate contrasting techniques such as

phase contrast, DIC and brightfield.

 

Illumination:

Halogen and Mercury Arc Lamp

 

Objectives:

N PLAN 5x/0.12

HC PL FLUOTAR 10x/0.30 (PH 1)

N PLAN L 20x/0.40 (PH 1; CORR)

HCX PL FLUOTAR 40x/0.6 (PH 2; CORR)

HCX PL APO 40x/1.25-0.75 (O)

PL APO 100x/1.40-0.7 (O)

 

Cameras:

Hamamatsu ORCA ER (monochrome)

Q-Imaging MicroPublisher 5.0 RTV

 

Filter Cubes: DAPI, FITC, TRITC, Cy3

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Software: Perkin Elmer Volocity

 

Stage: Motorized ASI stage

 

Enviromental Control: LCI- CU 301 incubation (temp)

PTI RatioMaster

 

Description:

PTI RatioMaster is a monochromator-based wide-field microscope system for measuring

ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations.

Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and

cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence

eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye

loading, non-uniform field of view, making ratio-fluorescence the most accurate method to

determine intracellular ion concentrations. More importantly, PTI RatioMaster is fast enough to

provide essential dynamic information about ion concentrations and interactions with other

molecules, drugs or added reagents. The PTI RatioMaster is capable of dynamic ratio fluorescence

measurements on a millisecond timescale. A xenon arc lamp provides high intensity, continuous

broadband illumination.

ArcturusXT LCM (laser cutter)

 

Description:

The Applied Biosystems™ ArcturusXT™ LCM System on a Nikon Eclipse Ti microscope is a unique

microdissection system that combines the power of infrared (IR) laser capture and ultraviolet (UV)

laser cutting in one modular platform. The solid-state IR laser delivers a gentle capture technique

that preserves the overall biomolecular integrity of cells, and is ideal for both single cells and

small numbers of cells. The solid-state UV laser permits superior speed and precision, and is well

suited for microdissecting dense tissue structures, and capturing large numbers of cells. This

unique combination of two lasers in one system helps ensure that sample custody is maintained

at all times, thereby enabling optimum isolation of DNA/RNA for downstream applications even in

a heterogeneous cell population.

Faculty of Dentistry

University of Toronto

124 Edward Street

4th floor

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