Confocal Systems

SAMPLE PREPARATION IS CRUCIAL IN CONFOCAL IMAGING.

PLEASE CONSULT WITH FACILITY STAFF FOR PROPER PROTOCOL.

Abberior STEDYCON

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NEW!!

This super-resolution system features a compact Nanoscope, STEDYCON, capable of achieving lateral STED resolution of <40nm, depending on dyes used. Mounted on an inverted Leica DMI6000B microscope, STEDYCON upgrades this to both confocal microscope with four channels (405nm, 488nm, 561nm, 640nm) and multicolor STED (775nm depletion) system! Patent protected QUAD-beam scanning technology, coupled with quasi-simultaneous line-interleaved scanning, allows fast acquisition speeds of up to 1.1 frames/s @ 512x512 pixels (line frequency up to 800Hz also between STED and confocal).

Capabilities:

  • Inverted configuration

  • Motorized XY scanning stage

  • Software controlled z-focusing piezo-drive

  • Avalanche photo detectors (APDs) have a superior quantum efficiency of up to 69% (@ 650nm) in the red fluorescence regime, and are able to collect plenty of photons for a meaningful image even when signal-levels are low. 

  • Detection: 500-550nm; 580-630nm; 650-700nm; line multiplexed into four channels

  • QUAD beam scanner: FOV of 90µm x 80µm (with a 100x objective)

  • Pinhole: motorized pinhole with 12 different pinhole sizes

  • Easy-STED geometry: all laser lines aligned by design; maintenance free optical beam path

 

Illumination:

X-Cite LED Illumination System – For quick visual assessment of fluorescent staining.

Excitation laser lines: 405nm(cw), 488nm, 561nm, 640nm (all pulsed)

STED laser: 775nm (pulsed)

 

Filter cubes: FITC/GFP, TRITC/RFP, VBG

 

Objective validated for STEDYCON: HCX PL APO 100x/1.40-0.70 OIL

 

Software: Browser-based control software with simple definition of z-stacks, acquisition queues, time series, and auto-save function

 

Active vibration isolation platform - Tested for STEDYCON.

Zeiss LSM 880 with Airyscan FAST

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Zeiss Axio Observer 7 equipped with LSM 880 scan head and Airyscan FAST module. With Airyscan you will be increasing the resolution of your imaging far beyond that of a classic confocal point scanning microscope. You can resolve 140 nm laterally and 400 nm axially, at 488 nm – without sacrificing sensitivity.

LSM 880 with Airyscan delivers up to 27 fps at 480x480 pixels in Fast mode, thus giving high acquisi­tion speed, sensitivity and superresolution that you need to answer your scientific questions.

Capabilities:

  • Inverted configuration

  • Live cell imaging

  • Motorized Piezo Z scanning stage

  • Fast superresolution and sensitive confocal image acquisition

  • Novel detector design enables a 4-8× improvement in signal-to-noise ratio (SNR) as compared to imaging with conventional confocal GaAsP detectors

  • 3D z-stacks

  • Stitching and tiling

  • Multi-position time lapse

  • Quantitative imaging

Lasers:

  • Argon Multiline 25 mW laser: 458nm, 488nm, 514nm

  • Diode laser: 405nm

  • DPSS laser: 561nm

  • HeNe laser: 633nm

 

Objectives:

  • PApo 10x/0.45

  • PApo 20x/0.8

  • Plan-APO 40x/1.40 Oil DIC

  • Plan-Apochromat 63x/1.40 Oil DIC

Detectors:

  • Spectral detector 32 ch GaAsP PMT+ 2 ch

Software:

  • Zen Black 2.3 with Airyscan module

Environmental Control:

  • Stage-top incubator PM S1 with various Lab Tek heating inserts.

Zeiss LSM 800 

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Zeiss Axio Observer 7 equipped with LSM 800 scan head.

Capabilities:

  •   Inverted configuration

  •   Live cell imaging

  •   Motorized Piezo Z scanning stage

  •   Novel detector design enables a 4-8× improvement in signal-to-noise ratio (SNR) as compared to imaging with conventional confocal GaAsP detectors

  •   3D z-stacks

  •   Stitching and tiling

  •   Multi-position time lapse

  •   Quantitative imaging

 

Lasers:

  • Diode laser 405nm, 5mW

  • Diode laser 488nm,10mW

  • Diode laser (SHG) 561nm, 10mW

  • Diode laser 640nm, 5mW

 

Objectives:

  • PApo 10x/0.45

  • PApo 20x/0.8

  • Plan-APO 40x/1.40 Oil DIC

  • Plan-Apochromat 63x/1.40 Oil DIC

 

Detectors:

  • 2 GaAsP PMT with a variable splitter dichroic

 

Software:

  • Zen Blue 2.6

 

Environmental Control:

  • Stage-top incubator PM S1 with various Lab Tek heating inserts.

Leica Confocal SP8

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Leica DMI6000 inverted microscope base equipped with Leica TCS SP8 confocal scanning platform.

Capabilities:

  •   Live cell imaging

  •   Motorized SuperZ Galvo scanning stage

  •   Highly sensitive GaAsP HyD detectors

  •   Quantitative imaging

  •   Spectrally tunable emission bands

  •   3D z-stacks

  •   Stitching and tiling

  •   Multi-position time lapse

Lasers:

  • Diode lasers: 405 nm, 448 nm, 638 nm

  • OPSL lasers: 488 nm, 552 nm

Objectives:

  • HCX PL APO CS 10x/0.40

  • HC PL APO CS2 20x/0.75

  • HC PL APO CS2 40x/0.1.30 Oil

  • HC PL APO CS2 63x/0.1.40 Oil

  • HC PL APO CS2 100x/0.1.40 Oil

 

Detectors:

  • Five channels - two PMTs, two HyDs - high sensitivity Hybrid Detectors, one PMT Trans - HyD1 | PMT2 | HyD3 | PMT4 | PMT Trans

 

Software:

  • Leica LAS X

 

Environmental Control:

  • Tokai Hit stage top incubator (with temp/CO2 controller)

With the exception of STEDYCON system, all others possess live imaging capabilities!

In terms of spatial resolution, you will always be limited by Abbe's diffraction limit ~ 250nm.